Review



psems tom20 halotag  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Addgene inc psems tom20 halotag
    Psems Tom20 Halotag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psems+tom20+halotag/pSEMS-Tom20-Halo7Tag+(Plasmid+%23111135)/pmc10278152-445-9-10
    Average 91 stars, based on 4 article reviews
    psems tom20 halotag - by Bioz Stars, 2026-09
    91/100 stars

    Images

    Related Articles

    Generated:

    Article Title: The endoplasmic reticulum adopts two distinct forms of tubules
    Article Snippet: .. Rtn4b-HaloTag was generated by inserting PCR-amplified Rtn4b from Rtn4b-GFP 362 and PCR-amplified HaloTag from pSEMS-Tom20-HaloTag (Addgene plasmid #111135; a gift from Karin Busch) 363 into the pcDNA3.1(+) backbone using Gibson Assembly. mCherry-Climp63(2xlumen) was prepared by assembling 364 the full-length mCherry-Climp63(mouse) with the luminal part of mouse Climp63 (AA110-AA575). ..

    Article Title: The endoplasmic reticulum adopts two distinct tubule forms
    Article Snippet: Rtn4b-GFP was generated by inserting two PCR-amplified fragments from the initial part (RTN4 AA1-AA185) and the later part (RTN4 AA1005-AA1192 plus AcGFP1) of Rtn4a-GFP into the pcDNA3.1(+) backbone using Gibson Assembly (New England Biolabs, #E2611S). .. Rtn4b-HaloTag was generated by inserting PCR-amplified Rtn4b from Rtn4b-GFP and PCR-amplified HaloTag from pSEMS-Tom20-HaloTag (Addgene plasmid #111135; a gift from Karin Busch) into the pcDNA3.1(+) backbone using Gibson Assembly. mCherry-Climp63(2xlumen) was prepared by assembling the full-length mCherry-Climp63(mouse) with the luminal part of mouse Climp63 (AA110-AA575). ..

    Polymerase Chain Reaction:

    Article Title: The endoplasmic reticulum adopts two distinct forms of tubules
    Article Snippet: .. Rtn4b-HaloTag was generated by inserting PCR-amplified Rtn4b from Rtn4b-GFP 362 and PCR-amplified HaloTag from pSEMS-Tom20-HaloTag (Addgene plasmid #111135; a gift from Karin Busch) 363 into the pcDNA3.1(+) backbone using Gibson Assembly. mCherry-Climp63(2xlumen) was prepared by assembling 364 the full-length mCherry-Climp63(mouse) with the luminal part of mouse Climp63 (AA110-AA575). ..

    Article Title: The endoplasmic reticulum adopts two distinct tubule forms
    Article Snippet: Rtn4b-GFP was generated by inserting two PCR-amplified fragments from the initial part (RTN4 AA1-AA185) and the later part (RTN4 AA1005-AA1192 plus AcGFP1) of Rtn4a-GFP into the pcDNA3.1(+) backbone using Gibson Assembly (New England Biolabs, #E2611S). .. Rtn4b-HaloTag was generated by inserting PCR-amplified Rtn4b from Rtn4b-GFP and PCR-amplified HaloTag from pSEMS-Tom20-HaloTag (Addgene plasmid #111135; a gift from Karin Busch) into the pcDNA3.1(+) backbone using Gibson Assembly. mCherry-Climp63(2xlumen) was prepared by assembling the full-length mCherry-Climp63(mouse) with the luminal part of mouse Climp63 (AA110-AA575). ..

    Plasmid Preparation:

    Article Title: The endoplasmic reticulum adopts two distinct forms of tubules
    Article Snippet: .. Rtn4b-HaloTag was generated by inserting PCR-amplified Rtn4b from Rtn4b-GFP 362 and PCR-amplified HaloTag from pSEMS-Tom20-HaloTag (Addgene plasmid #111135; a gift from Karin Busch) 363 into the pcDNA3.1(+) backbone using Gibson Assembly. mCherry-Climp63(2xlumen) was prepared by assembling 364 the full-length mCherry-Climp63(mouse) with the luminal part of mouse Climp63 (AA110-AA575). ..

    Article Title: The endoplasmic reticulum adopts two distinct tubule forms
    Article Snippet: Rtn4b-GFP was generated by inserting two PCR-amplified fragments from the initial part (RTN4 AA1-AA185) and the later part (RTN4 AA1005-AA1192 plus AcGFP1) of Rtn4a-GFP into the pcDNA3.1(+) backbone using Gibson Assembly (New England Biolabs, #E2611S). .. Rtn4b-HaloTag was generated by inserting PCR-amplified Rtn4b from Rtn4b-GFP and PCR-amplified HaloTag from pSEMS-Tom20-HaloTag (Addgene plasmid #111135; a gift from Karin Busch) into the pcDNA3.1(+) backbone using Gibson Assembly. mCherry-Climp63(2xlumen) was prepared by assembling the full-length mCherry-Climp63(mouse) with the luminal part of mouse Climp63 (AA110-AA575). ..

    Transfection:

    Article Title: Organelle interactions compartmentalize hepatic fatty acid trafficking and metabolism
    Article Snippet: Cells were countered stained for mitochondria using MitoTracker Deep Red (Thermo) and AutoDOT neutral lipid stain (mondansylpentane [MDH] Lipid Droplet Staining Tool; Absepta) 20 min prior to imaging. .. For the SMLM studies, AML12 cells were transfected with pSEMS-TOM20-HaloTag (Addgene#111135) using Qiagen’s Effectine transfection reagent. ..

    Article Title: A Detailed Evaluation of the Effect of Prostate-specific Antigen-based Screening on Morbidity and Mortality of Prostate Cancer: 21-year Follow-up Results of the Rotterdam Section of the European Randomised Study of Screening for Prostate Cancer.
    Article Snippet: Cells were countered stained for mitochondria using MitoTracker Deep Red (Thermo) and AutoDOT neutral lipid stain (mondansylpentane [MDH] Lipid Droplet Staining Tool; Absepta) 20 min prior to imaging. .. For the SMLM studies, AML12 cells were transfected with pSEMS-TOM20-HaloTag (Addgene#111135) using Qiagen’s Effectine transfection reagent. ..



    Similar Products

    91
    Addgene inc psems tom20 halotag
    Psems Tom20 Halotag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psems+tom20+halotag/pSEMS-Tom20-Halo7Tag+(Plasmid+%23111135)/pmc10278152-445-9-10
    Average 91 stars, based on 1 article reviews
    psems tom20 halotag - by Bioz Stars, 2026-09
    91/100 stars
      Buy from Supplier

    Image Search Results